{"id":695,"date":"2015-06-15T14:11:55","date_gmt":"2015-06-15T12:11:55","guid":{"rendered":"http:\/\/www.dbsv.uninsubria.it\/dbsv\/?page_id=695"},"modified":"2019-04-01T14:07:51","modified_gmt":"2019-04-01T12:07:51","slug":"genetica-molecolare","status":"publish","type":"page","link":"https:\/\/www.dbsv.uninsubria.it\/dbsv\/?page_id=695","title":{"rendered":"Molecular Genetics Lab"},"content":{"rendered":"<h3 style=\"text-align: center;\"><span style=\"color: #ff6600;\"><strong>Understanding the genetic bases of human disease<\/strong><\/span><\/h3>\n<p style=\"text-align: center;\"><img decoding=\"async\" src=\"http:\/\/www.dbsv.uninsubria.it\/dbsv\/wp-content\/uploads\/2015\/06\/fig1.jpg\" alt=\"Notte dei ricercatori\" \/><\/p>\n<h6 style=\"text-align: center;\"><em>Notte dei Ricercatori (Researcher\u2019s night), 2014<\/em><\/h6>\n<h4 style=\"text-align: center;\"><span style=\"color: #ff6600;\"><strong>People<\/strong><\/span><\/h4>\n<ul>\n<li style=\"list-style-type: none;\">\n<ul>\n<li style=\"text-align: left;\">\n<h6>Prof. <a href=\"https:\/\/www.uninsubria.it\/hpp\/paola.campomenosi\">Paola Campomenosi<\/a>,\u00a0\u00a0e-mail to <a href=\"mailto:paola.campomenosi@uninsubria.it\"><em><u>paola.campomenosi@uninsubria.it<\/u><\/em><\/a><\/h6>\n<\/li>\n<\/ul>\n<\/li>\n<\/ul>\n<ul>\n<li style=\"list-style-type: none;\">\n<ul>\n<li style=\"text-align: left;\">\n<h6>Dr.ssa <a href=\"https:\/\/www.uninsubria.it\/hpp\/raffaella.cinquetti\" target=\"_blank\" rel=\"noopener noreferrer\">Raffaella Cinquetti<\/a>, e-mail to\u00a0<a href=\"mailto:raffaella.cinquetti@uninsubria.it\"><em><u>raffaella.cinquetti@uninsubria.it<\/u><\/em><\/a><\/h6>\n<\/li>\n<\/ul>\n<\/li>\n<\/ul>\n<ul>\n<li style=\"text-align: left;\">\n<h6>Dr.ssa<a href=\"https:\/\/www.uninsubria.it\/hpp\/sarah.grossi\" target=\"_blank\" rel=\"noopener noreferrer\"> Sarah Grossi<\/a>, e-mail to <a href=\"mailto:sarah.grossi@uninsubria.it\"><em><u>sarah.grossi@uninsubria.it<\/u><\/em><\/a><\/h6>\n<\/li>\n<\/ul>\n<p><span style=\"font-size: large;\">We are a small, very collaborative group: from collaboration we can get the best ideas and complement expertises.<\/span><\/p>\n<p><span style=\"font-size: large;\">Our research is devoted to understanding the role of sequence variation or of changes in gene regulation in human disease. We study the <span style=\"color: #33cccc;\"><span style=\"color: #ff6600;\">role of gene products and the mechanisms underlying their malfunctioning in specific diseases<span style=\"color: #000000;\">.<\/span><\/span><\/span>\u00a0This in turn can lead to their exploitation in diagnosis, prognosis or in finding cures.<\/span><\/p>\n<p><span style=\"font-size: large;\">Here is a description of the ongoing projects, but keep in touch\u2026 We are planning more!<\/span><\/p>\n<h6 style=\"text-align: center;\"><span style=\"color: #ff6600;\">1. <em>Characterization of Proline dehydrogenase, an<\/em><br \/>\n<em> enzyme involved in tumorigenesis and schizophrenia.<\/em><\/span><\/h6>\n<table width=\"732\">\n<tbody>\n<tr>\n<td style=\"text-align: center;\" width=\"50%\"><a href=\"http:\/\/www.dbsv.uninsubria.it\/dbsv\/wp-content\/uploads\/2015\/06\/Fig2.jpg\"><img loading=\"lazy\" decoding=\"async\" class=\"alignnone size-medium wp-image-782\" src=\"http:\/\/www.dbsv.uninsubria.it\/dbsv\/wp-content\/uploads\/2015\/06\/Fig2-300x194.jpg\" alt=\"Fig2\" width=\"300\" height=\"194\" srcset=\"https:\/\/www.dbsv.uninsubria.it\/dbsv\/wp-content\/uploads\/2015\/06\/Fig2-300x194.jpg 300w, https:\/\/www.dbsv.uninsubria.it\/dbsv\/wp-content\/uploads\/2015\/06\/Fig2.jpg 958w\" sizes=\"auto, (max-width: 300px) 100vw, 300px\" \/><\/a><\/p>\n<p style=\"text-align: left;\"><span style=\"font-size: medium;\"> <span style=\"font-size: large;\">Study of the regulation of the <em>PRODH <\/em>gene during cellular stress and identification of the factors involved in this type of regulation can help to understand the biological function of this enzyme, and how it is involved in the decision of cell fate between survival and apoptosis.<\/span><\/span><\/p>\n<p style=\"text-align: left;\"><em><strong>Collaboration\u00a0<\/strong> with\u00a0 Prof.\u00a0 E. Monti and M. Gariboldi (<a href=\"http:\/\/www4.uninsubria.it\/on-line\/home\/naviga-per-tema\/didattica\/dipartimenti\/dipartimento-di-scienze-teoriche-e-applicate---dista.html\" target=\"_blank\" rel=\"noopener noreferrer\">DiSTA<\/a>)\u00a0;\u00a0Prof.\u00a0 L. Pollegioni\u00a0 and\u00a0 S. \u00a0Sacchi\u00a0(<a href=\"http:\/\/www4.uninsubria.it\/on-line\/home\/naviga-per-tema\/didattica\/dipartimenti\/dipartimento-di-biotecnologie-e-scienze-della-vita---dbsv.html\" target=\"_blank\" rel=\"noopener noreferrer\">DBSV<\/a>).<\/em><\/p>\n<\/td>\n<td style=\"text-align: center;\" width=\"50%\"><a href=\"http:\/\/www.dbsv.uninsubria.it\/dbsv\/wp-content\/uploads\/2015\/06\/Fig3.jpg\"><img loading=\"lazy\" decoding=\"async\" class=\"alignnone wp-image-783\" src=\"http:\/\/www.dbsv.uninsubria.it\/dbsv\/wp-content\/uploads\/2015\/06\/Fig3-300x214.jpg\" alt=\"Fig3\" width=\"218\" height=\"155\" srcset=\"https:\/\/www.dbsv.uninsubria.it\/dbsv\/wp-content\/uploads\/2015\/06\/Fig3-300x214.jpg 300w, https:\/\/www.dbsv.uninsubria.it\/dbsv\/wp-content\/uploads\/2015\/06\/Fig3.jpg 814w\" sizes=\"auto, (max-width: 218px) 100vw, 218px\" \/><\/a><\/p>\n<p style=\"text-align: left;\"><span style=\"font-size: large;\">We mapped PRODH staining in human normal tissues or tumours by immunohistochemistry. We are now characterizing its expression in lung cancer (NSCLC). Our preliminary data suggest its possible exploitation for differential diagnosis of adenocarcinoma and squamocellular carcinoma.<\/span><\/p>\n<p style=\"text-align: left;\"><span style=\"font-size: small;\"><em><strong>Collaboration <\/strong>with Prof. Fausto Sessa and Anna Maria Chiaravalli (<a href=\"http:\/\/www4.uninsubria.it\/on-line\/home\/naviga-per-tema\/didattica\/dipartimenti\/dipartimento-di-scienze-chirurgiche-e-morfologiche---dscm.html\" target=\"_blank\" rel=\"noopener noreferrer\">DSCM<\/a>, Anatomopathology).<\/em><\/span><\/p>\n<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n<h6 style=\"text-align: center;\"><em><strong><span style=\"color: #ff6600;\">2. Quantification of circulating microRNAs as\u00a0biomarkers for early diagnosis of lung cancer.<\/span><\/strong><\/em><\/h6>\n<p style=\"text-align: center;\"><img decoding=\"async\" src=\"http:\/\/www.dbsv.uninsubria.it\/dbsv\/wp-content\/uploads\/2015\/06\/fig6.jpg\" alt=\"PCR\" \/><\/p>\n<p><span style=\"font-size: large;\">Lung cancer is one of the most common cancers, leading to death primarily because its diagnosis occurs late, when tumours are at advanced stages. Therefore, this type of tumour would greatly benefit from an early diagnosis. MicroRNAs are small RNAs, regulating gene expression, that have been shown to circulate in the blood and whose levels are altered in several human diseases. We are conducting a pilot case-control study, quantifying specific microRNAs in sera of stage I or II NSCLC patients or matched controls by Digital PCR.<\/span><\/p>\n<p><span style=\"font-size: small;\"><em><strong>Collaboration<\/strong><span style=\"color: #000000;\"> with\u00a0Prof.\u00a0 Lorenzo\u00a0Dominioni\u00a0 (<a href=\"http:\/\/www4.uninsubria.it\/on-line\/home\/naviga-per-tema\/didattica\/dipartimenti\/dipartimento-di-scienze-chirurgiche-e-morfologiche---dscm.html\" target=\"_blank\" rel=\"noopener noreferrer\">DSCM<\/a>,\u00a0 Universit\u00e0\u00a0 dell\u2019Insubria),\u00a0 Douglas\u00a0Noonan\u00a0(<a href=\"http:\/\/www4.uninsubria.it\/on-line\/home\/naviga-per-tema\/didattica\/dipartimenti\/dipartimento-di-biotecnologie-e-scienze-della-vita---dbsv.html\" target=\"_blank\" rel=\"noopener noreferrer\">DBSV<\/a>,\u00a0 Universit\u00e0\u00a0dell\u2019Insubria) and Dr. Elisabetta Gini (PhD student); Prof. Albino Poli\u00a0(Universit\u00e0\u00a0di\u00a0Verona).<\/span><\/em><\/span><\/p>\n<h6 style=\"text-align: center;\"><span style=\"color: #ff6600;\"><em>3. Expression of cytokines and matrix degrading enzymes by cells grown on artificial or biological materials for abdominal wall repair<\/em><\/span><img decoding=\"async\" src=\"http:\/\/www.dbsv.uninsubria.it\/dbsv\/wp-content\/uploads\/2015\/06\/fig4.jpg\" alt=\"Matrix\" \/><\/h6>\n<p><span style=\"font-size: large;\">Meshes are materials used to reinforce the abdominal wall during surgery for hernia repair. They can be either synthetic or derived from decellularized biological material from different sources (e.g. porcine derma or intestinal submucosa). Their interaction with the host will determine the success of the implant. This project aims at characterizing morphology and expression of <span style=\"color: #ff6600;\">cytokines, matrix degrading enzymes and myofibroblast markers<\/span> in vitro by the main cell types physiologically present at the site of implant, namely macrophages and fibroblasts. We are characterizing gene and protein expression after growth of these cells on the different materials.<\/span><\/p>\n<p style=\"text-align: left;\"><span style=\"font-size: small;\"><em><strong>Collaboration<\/strong>\u00a0with\u00a0Prof.\u00a0Giampiero\u00a0Campanelli\u00a0(<a href=\"http:\/\/www4.uninsubria.it\/on-line\/home\/naviga-per-tema\/didattica\/dipartimenti\/dipartimento-di-scienze-chirurgiche-e-morfologiche---dscm.html\" target=\"_blank\" rel=\"noopener noreferrer\">DSCM<\/a>,\u00a0University\u00a0of\u00a0Insubria),\u00a0Annalisa\u00a0Grimaldi\u00a0(<a href=\"http:\/\/www4.uninsubria.it\/on-line\/home\/naviga-per-tema\/didattica\/dipartimenti\/dipartimento-di-biotecnologie-e-scienze-della-vita---dbsv.html\" target=\"_blank\" rel=\"noopener noreferrer\">DBSV<\/a>, University of Insubria).<\/em><\/span><\/p>\n<h4 style=\"text-align: center;\"><span style=\"color: #ff6600;\"><strong>Papers<\/strong><\/span><\/h4>\n<ol>\n<li>Moretti F.<span class=\"\">,\u00a0<b class=\"\">D\u2019Antona<\/b><\/span><sup class=\"\"><b class=\"\">\u00a0<\/b><\/sup><span class=\"\"><b class=\"\">P<\/b>., Finardi E., Barbetta<\/span><sup class=\"\">\u00a0<\/sup><span class=\"\">M, Dominioni<\/span><sup class=\"\">\u00a0<\/sup><span class=\"\">L., Poli<\/span><sup class=\"\">\u00a0<\/sup><span class=\"\">A., Gini<\/span><sup class=\"\">\u00a0<\/sup><span class=\"\">E.,\u00a0<b class=\"\">Noonan D.M.<\/b>, Imperatori<\/span><span class=\"\">A., Rotolo<\/span><sup class=\"\">\u00a0<\/sup><span class=\"\">N., Cattoni<\/span><sup class=\"\">\u00a0<\/sup><span class=\"\">M.\u00a0\u00a0<\/span><span class=\"\"><b class=\"\">Campomenosi P. <\/b>2017.<b class=\"\">\u00a0<\/b><\/span><span class=\"\">Systematic review and critique of circulating miRNAs as biomarkers of stage I-II non-small cell lung cancer.\u00a0<\/span><i class=\"\"><span class=\"\" lang=\"IT\">Oncotarget.\u00a0<\/span><\/i><span class=\"\" lang=\"IT\">https:\/\/doi.org\/10.18632\/oncotarget.21739\u00a0<\/span><\/li>\n<li>Guarino MP, Altomare A, Barera S, Locato V, Cocca S, Franchin C, Arrigoni G,\u00a0<b class=\"\">Vannini C, Grossi S, Campomenosi P<\/b>, Pasqualetti V,\u00a0<b class=\"\">Bracale M<\/b>, Alloni R, De Gara L, Cicala M. 2017. Effect of Inulin on Proteome Changes Induced by Pathogenic Lipopolysaccharide in Human Colon.\u00a0<span class=\"\">PLoS One. Jan 9;12(1):e0169481.<\/span><span class=\"\">\u00a0<\/span><\/li>\n<li>Guarino MPL, Vannini C, Altomare A, Barera S, Locato V, Cocca S, Arrigoni G, Alloni R, De Gara L, <strong>Campomenosi P<\/strong>, Cicala M. 2016. Tu1851 Protective Effect of Inulin on LPS-Induced Intestinal Smooth Muscle Impairment: A Proteomic Approach. Gastroenterology, 150(4):S960. http:\/\/dx.doi.org\/10.1016\/S0016-5085(16)3<\/li>\n<li><strong>Campomenosi P<\/strong>, Gini E, Noonan DM, Poli A, D&#8217;Antona P, Rotolo N, Dominioni L, Imperatori A. 2016.\u00a0 A comparison between quantitative PCR and droplet digital PCR technologies for circulating microRNA quantification in human lung cancer.\u00a0BMC Biotechnol,\u00a016(1):60. doi: 10.1186\/s12896-016-0292-7.<\/li>\n<li>Gallo S, Grossi S, Montrasio G,\u00a0<b class=\"\">Binelli G<\/b>,\u00a0<b class=\"\">Cinquetti R<\/b>, Simmen D,\u00a0<b class=\"\">Castelnuovo P,<\/b>\u00a0<b class=\"\">Campomenosi P.<\/b>\u00a02016. <em>TAS2R38<\/em> taste receptor gene and chronic rhinosinusitis: new data from an Italian population. BMC Med Genet. 2016 Aug 11;17(1):54. doi:10.1186\/s12881-016-0321-3.<\/li>\n<li><span class=\"\" lang=\"IT\"><b class=\"\">Vannini C., Bracale<sup class=\"\">\u00a0<\/sup>M.<\/b>, Crinelli R., Mastrogiacomo A.R.,<b class=\"\">Campomenosi P<\/b>.,\u00a0<b class=\"\">Marsoni<sup class=\"\">\u00a0<\/sup>M<\/b>., Scoccianti<sup class=\"\">\u00a0<\/sup>V. (2014).<\/span><span class=\"\" lang=\"IT\">Proteomic analysi<\/span><span class=\"\">s of MG132-treated germinating pollen reveals expression signatures associated with proteasome inhibition.<\/span><i class=\"\"><span class=\"\">\u00a0<\/span><\/i><i class=\"\"><span class=\"\" lang=\"IT\">PLoS One<\/span><\/i><span class=\"\" lang=\"IT\">, 9(9): e108811.<\/span><\/li>\n<li>Monti P., Ciribilli Y., Bisio A., Foggetti G., Raimondi I., <strong>Campomenosi P<\/strong>., Menichini P., Fronza G., Inga A. (2014). \u0394N-P63\u03b1 and TA-P63\u03b1 exhibit intrinsic differences in transactivation specificities that depend on distinct features of DNA target sites. <em>Oncotarget<\/em>, 5, 2116-2130.<\/li>\n<li>Cappelletti P., <strong>Campomenosi P<\/strong>., Pollegioni L. and Sacchi S. (2014). On the degradation of human D-amino acid oxidase and its interacting partner pLG72, two key proteins in D-serine catabolism in the brain. <em>FEBS Journal<\/em>, 281, 708-723.<\/li>\n<li>Raimondi I., Ciribilli Y., Monti P., Bisio A., De Sanctis V., Pollegioni L., Fronza G.,\u00a0 Inga A. and <strong>Campomenosi <\/strong>P. (2013). P53 family members modulate the expression of PRODH, but not PRODH2, via intronic p53 response elements, <em>PLoS One<\/em>, 8(7): e69152.<\/li>\n<li><strong>Campomenosi P<\/strong>., <strong>Cinquetti R<\/strong>., Tallarita E., Lindqvist C., Raimondi I., Grassi P., N\u00e4sman J., Dell A., Haslam S.M., Taramelli R. and Acquati F. (2011). Comparison of the baculovirus insect cell and Pichia pastoris heterologous systems for the expression of the human tumor suppressor protein RNASET2. <em>Biotechnology and Applied Biochemistry<\/em>, 58:\u00a039\u201349.<\/li>\n<li>Acquati F., Bertilaccio S., Grimaldi A., Monti L., <strong>Cinquetti R<\/strong>., Bonetti P., Lualdi M., Vidalino L., Fabbri M., Sacco M.G., Rooijen N., <strong>Campomenosi P<\/strong>., Vigetti D., Passi A., Riva C., Capella C., Sanvito F., Doglioni C., Gribaldo L., Macchi P., Sica A., Noonan D.M., Ghia P. and Taramelli R. (2011). Microenvironmental control of malignancy exerted by RNASET2, a widely conserved extracellular RNase. <em>Proc Natl Acad Sci U. S. A.<\/em> 108, 1104-1109.<\/li>\n<\/ol>\n","protected":false},"excerpt":{"rendered":"<p>Understanding the genetic bases of human disease Notte dei Ricercatori (Researcher\u2019s night), 2014 People Prof. Paola Campomenosi,\u00a0\u00a0e-mail to paola.campomenosi@uninsubria.it Dr.ssa Raffaella Cinquetti, e-mail to\u00a0raffaella.cinquetti@uninsubria.it Dr.ssa Sarah Grossi, e-mail to sarah.grossi@uninsubria.it We are a small, very collaborative group: from collaboration we can get the best ideas and complement expertises. Our research is devoted to understanding the &hellip; <a href=\"https:\/\/www.dbsv.uninsubria.it\/dbsv\/?page_id=695\" class=\"more-link\">Continua a leggere <span class=\"screen-reader-text\">Molecular Genetics Lab<\/span><\/a><\/p>\n","protected":false},"author":1,"featured_media":0,"parent":0,"menu_order":0,"comment_status":"closed","ping_status":"closed","template":"","meta":{"footnotes":""},"class_list":["post-695","page","type-page","status-publish","hentry"],"_links":{"self":[{"href":"https:\/\/www.dbsv.uninsubria.it\/dbsv\/index.php?rest_route=\/wp\/v2\/pages\/695","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/www.dbsv.uninsubria.it\/dbsv\/index.php?rest_route=\/wp\/v2\/pages"}],"about":[{"href":"https:\/\/www.dbsv.uninsubria.it\/dbsv\/index.php?rest_route=\/wp\/v2\/types\/page"}],"author":[{"embeddable":true,"href":"https:\/\/www.dbsv.uninsubria.it\/dbsv\/index.php?rest_route=\/wp\/v2\/users\/1"}],"replies":[{"embeddable":true,"href":"https:\/\/www.dbsv.uninsubria.it\/dbsv\/index.php?rest_route=%2Fwp%2Fv2%2Fcomments&post=695"}],"version-history":[{"count":110,"href":"https:\/\/www.dbsv.uninsubria.it\/dbsv\/index.php?rest_route=\/wp\/v2\/pages\/695\/revisions"}],"predecessor-version":[{"id":3925,"href":"https:\/\/www.dbsv.uninsubria.it\/dbsv\/index.php?rest_route=\/wp\/v2\/pages\/695\/revisions\/3925"}],"wp:attachment":[{"href":"https:\/\/www.dbsv.uninsubria.it\/dbsv\/index.php?rest_route=%2Fwp%2Fv2%2Fmedia&parent=695"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}