{"id":2,"date":"2017-06-12T11:17:34","date_gmt":"2017-06-12T09:17:34","guid":{"rendered":"http:\/\/www.dbsv.uninsubria.it\/nbiomol\/?page_id=2"},"modified":"2017-06-23T15:29:18","modified_gmt":"2017-06-23T13:29:18","slug":"pagina-di-esempio","status":"publish","type":"page","link":"https:\/\/www.dbsv.uninsubria.it\/nbiomol\/?page_id=2","title":{"rendered":"Ongoing projects"},"content":{"rendered":"<ul>\n<li>Using a platform of primary cultures of Cdkl5-deficient neurons we are searching for drugs that are capable of reverting the morphological and molecular defects caused by loss of Cdkl5. While we continue searching for new drugs we have already identified two compounds that are capable of normalizing various parameters of Cdkl5-deficient neurons. These drugs will be further studied to fully understand their therapeutic potential.\u00a0<img loading=\"lazy\" decoding=\"async\" class=\"size-medium wp-image-164 aligncenter\" src=\"http:\/\/www.dbsv.uninsubria.it\/nbiomol\/wp-content\/uploads\/2017\/06\/Kilstrup3-300x101.jpg\" alt=\"\" width=\"600\" height=\"202\" srcset=\"https:\/\/www.dbsv.uninsubria.it\/nbiomol\/wp-content\/uploads\/2017\/06\/Kilstrup3-300x101.jpg 300w, https:\/\/www.dbsv.uninsubria.it\/nbiomol\/wp-content\/uploads\/2017\/06\/Kilstrup3-768x260.jpg 768w, https:\/\/www.dbsv.uninsubria.it\/nbiomol\/wp-content\/uploads\/2017\/06\/Kilstrup3-1024x346.jpg 1024w\" sizes=\"auto, (max-width: 600px) 100vw, 600px\" \/><\/li>\n<li>Among the molecular networks regulated by CDKL5, we are investigating its role in controlling cytoskeleton dynamics in proliferating cells and post-mitotic neurons.\u00a0\u00a0\u00a0<img loading=\"lazy\" decoding=\"async\" class=\"size-medium wp-image-165 alignleft\" src=\"http:\/\/www.dbsv.uninsubria.it\/nbiomol\/wp-content\/uploads\/2017\/06\/Kilstrup4a-179x300.jpg\" alt=\"\" width=\"179\" height=\"300\" srcset=\"https:\/\/www.dbsv.uninsubria.it\/nbiomol\/wp-content\/uploads\/2017\/06\/Kilstrup4a-179x300.jpg 179w, https:\/\/www.dbsv.uninsubria.it\/nbiomol\/wp-content\/uploads\/2017\/06\/Kilstrup4a-768x1285.jpg 768w, https:\/\/www.dbsv.uninsubria.it\/nbiomol\/wp-content\/uploads\/2017\/06\/Kilstrup4a-612x1024.jpg 612w\" sizes=\"auto, (max-width: 179px) 100vw, 179px\" \/>\u00a0 \u00a0\u00a0<img loading=\"lazy\" decoding=\"async\" class=\"size-medium wp-image-166 alignleft\" src=\"http:\/\/www.dbsv.uninsubria.it\/nbiomol\/wp-content\/uploads\/2017\/06\/Kilstrup4b-176x300.jpg\" alt=\"\" width=\"176\" height=\"300\" srcset=\"https:\/\/www.dbsv.uninsubria.it\/nbiomol\/wp-content\/uploads\/2017\/06\/Kilstrup4b-176x300.jpg 176w, https:\/\/www.dbsv.uninsubria.it\/nbiomol\/wp-content\/uploads\/2017\/06\/Kilstrup4b-768x1309.jpg 768w, https:\/\/www.dbsv.uninsubria.it\/nbiomol\/wp-content\/uploads\/2017\/06\/Kilstrup4b-601x1024.jpg 601w\" sizes=\"auto, (max-width: 176px) 100vw, 176px\" \/><\/li>\n<\/ul>\n<p>&nbsp;<\/p>\n<p>&nbsp;<\/p>\n<p>&nbsp;<\/p>\n<p>&nbsp;<\/p>\n<p>&nbsp;<\/p>\n<p>&nbsp;<\/p>\n<p><span style=\"font-size: 1rem;\">T<\/span><em style=\"font-size: 1rem;\">he microtubule plus-end binding protein CLIP170 fused to GFP was expressed in Cos7 cells silenced for CDKL5 and its association with microtubules analyzed through time-lapse imaging. The stability of the microtubule associaiton of\u00a0 CLIP170 is significantly reduced in the absence of CDKL5.<\/em><\/p>\n<ul>\n<li>CDKL5 plays an important role in regulating synaptic activity and spine maturation; we are particularly interested in understanding the molecular basis for these defects.<\/li>\n<\/ul>\n<p style=\"text-align: center;\">\u00a0<img loading=\"lazy\" decoding=\"async\" class=\"alignnone size-medium wp-image-175\" src=\"http:\/\/www.dbsv.uninsubria.it\/nbiomol\/wp-content\/uploads\/2017\/06\/Kilstrup20-300x149.jpg\" alt=\"\" width=\"600\" height=\"298\" srcset=\"https:\/\/www.dbsv.uninsubria.it\/nbiomol\/wp-content\/uploads\/2017\/06\/Kilstrup20-300x149.jpg 300w, https:\/\/www.dbsv.uninsubria.it\/nbiomol\/wp-content\/uploads\/2017\/06\/Kilstrup20-768x380.jpg 768w, https:\/\/www.dbsv.uninsubria.it\/nbiomol\/wp-content\/uploads\/2017\/06\/Kilstrup20.jpg 860w\" sizes=\"auto, (max-width: 600px) 100vw, 600px\" \/><\/p>\n<p><b><em>Left: <\/em><\/b><em>Cdkl5-deficient neurons are characterized by the presence of immature spines in correspondence with defects in synaptic plasticity. CDKL5 expression was silenced in primary hippocampal neurons with a shRNA against CDKL5 or as control shLacZ. The morphology of dendritic spines was analyzed after 18 days in culture.<\/em><\/p>\n<p><em><b>Right: <\/b>immunofluorescence showing pre- and post-synaptic markers in cultured hippocampal neurons (SYN1, red; GluR2, green).<\/em><\/p>\n","protected":false},"excerpt":{"rendered":"<p>Using a platform of primary cultures of Cdkl5-deficient neurons we are searching for drugs that are capable of reverting the morphological and molecular defects caused by loss of Cdkl5. While we continue searching for new drugs we have already identified two compounds that are capable of normalizing various parameters of Cdkl5-deficient neurons. These drugs will &hellip; <\/p>\n<p class=\"link-more\"><a href=\"https:\/\/www.dbsv.uninsubria.it\/nbiomol\/?page_id=2\" class=\"more-link\">Leggi tutto<span class=\"screen-reader-text\"> &#8220;Ongoing projects&#8221;<\/span><\/a><\/p>\n","protected":false},"author":1,"featured_media":0,"parent":0,"menu_order":0,"comment_status":"closed","ping_status":"closed","template":"","meta":{"footnotes":""},"class_list":["post-2","page","type-page","status-publish","hentry"],"_links":{"self":[{"href":"https:\/\/www.dbsv.uninsubria.it\/nbiomol\/index.php?rest_route=\/wp\/v2\/pages\/2","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/www.dbsv.uninsubria.it\/nbiomol\/index.php?rest_route=\/wp\/v2\/pages"}],"about":[{"href":"https:\/\/www.dbsv.uninsubria.it\/nbiomol\/index.php?rest_route=\/wp\/v2\/types\/page"}],"author":[{"embeddable":true,"href":"https:\/\/www.dbsv.uninsubria.it\/nbiomol\/index.php?rest_route=\/wp\/v2\/users\/1"}],"replies":[{"embeddable":true,"href":"https:\/\/www.dbsv.uninsubria.it\/nbiomol\/index.php?rest_route=%2Fwp%2Fv2%2Fcomments&post=2"}],"version-history":[{"count":20,"href":"https:\/\/www.dbsv.uninsubria.it\/nbiomol\/index.php?rest_route=\/wp\/v2\/pages\/2\/revisions"}],"predecessor-version":[{"id":200,"href":"https:\/\/www.dbsv.uninsubria.it\/nbiomol\/index.php?rest_route=\/wp\/v2\/pages\/2\/revisions\/200"}],"wp:attachment":[{"href":"https:\/\/www.dbsv.uninsubria.it\/nbiomol\/index.php?rest_route=%2Fwp%2Fv2%2Fmedia&parent=2"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}